fluorescent protein gfp_loxp_genscript Search Results


fluorescent protein gfp_loxp_genscript  (GenScript corporation)
90
GenScript corporation fluorescent protein gfp_loxp_genscript
Dual-marker cassettes facilitate tagging of genes with <t>fluorescent</t> protein transgenes or other epitopes. (A) Schematic of a dual-marker cassette his-72 :: GFP tagging vector. The selection cassette is housed within a constitutively spliced synthetic intron (dotted lines denote 5′ and 3′splice sites), such that after Cre -mediated excision of the cassette, the remaining loxP site will be spliced out of the mature GFP mRNA open reading frame, creating near seamless editing. (B) Fluorescent micrographs of endogenously tagged HIS-72::GFP (top) and HIS-72::mCherry (bottom). Scale bars, 100 µm.
Fluorescent Protein Gfp Loxp Genscript, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fluorescent protein gfp_loxp_genscript/product/GenScript corporation
Average 90 stars, based on 1 article reviews
fluorescent protein gfp_loxp_genscript - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


Dual-marker cassettes facilitate tagging of genes with fluorescent protein transgenes or other epitopes. (A) Schematic of a dual-marker cassette his-72 :: GFP tagging vector. The selection cassette is housed within a constitutively spliced synthetic intron (dotted lines denote 5′ and 3′splice sites), such that after Cre -mediated excision of the cassette, the remaining loxP site will be spliced out of the mature GFP mRNA open reading frame, creating near seamless editing. (B) Fluorescent micrographs of endogenously tagged HIS-72::GFP (top) and HIS-72::mCherry (bottom). Scale bars, 100 µm.

Journal: Genetics

Article Title: Efficient Genome Editing in Caenorhabditis elegans with a Toolkit of Dual-Marker Selection Cassettes

doi: 10.1534/genetics.115.180679

Figure Lengend Snippet: Dual-marker cassettes facilitate tagging of genes with fluorescent protein transgenes or other epitopes. (A) Schematic of a dual-marker cassette his-72 :: GFP tagging vector. The selection cassette is housed within a constitutively spliced synthetic intron (dotted lines denote 5′ and 3′splice sites), such that after Cre -mediated excision of the cassette, the remaining loxP site will be spliced out of the mature GFP mRNA open reading frame, creating near seamless editing. (B) Fluorescent micrographs of endogenously tagged HIS-72::GFP (top) and HIS-72::mCherry (bottom). Scale bars, 100 µm.

Article Snippet: The loxP sites were designed to reside in an empty vector (loxP_genscript) or in a synthetic strongly spliced intron sequence within fluorescent protein (GFP_loxP_genscript, mCherry_loxP_genscript, GFP::3xHA_loxP_genscript) and/or 3xHA (3xHA_loxP_genscript) coding sequences. (See Table S2 for relevant information on all vectors in our toolkit and their features.)

Techniques: Marker, Plasmid Preparation, Selection